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Cryostat HM525 NX Cryostat Service – Manual – English Cat. No. 391471 – Issue 2.0 / 2013

 Service Manual Cryostat HM525 NX Cat.No. 391471

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Thermo Fisher Scientific Issue 2.0 – 2013

Company Information © Copyright 2013. Thermo Fisher Scientific Inc. All rights reserved. Thermo Fisher Scientific Inc. (Thermo Fisher Scientific) is the world leader in serving science, offering a unique combination of innovative technologies. Thermo Scientific is a brand name of Thermo Fisher Scientific. All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries. Thermo Fisher Scientific makes every attempt to ensure that the information contained in this supporting document is correct and clearly stated, but does not accept responsibility for any errors or omissions. The development of Thermo Scientific products and services is an ongoing process. Please ensure that any published information you use as a reference is up to date and relates to the condition of the product. If necessary, check with your local Thermo Fisher Scientific representative. This document may not, in whole or in part, be copied, photocopied, reproduced, translated, or converted to any electronic or other form without prior written consent of Thermo Fisher Scientific. All information contained in this manual is proprietary and confidential, and the exclusive property of Thermo Fisher Scientific and is protected by copyright.

Contact Addresses:  Microm International, GmbH Subsidiary of Thermo Fisher Scientific Otto-Hahn-Strasse 1a 69190 Walldorf

 

Germany USA Distributor:  Richard-Allan Scientific Subsidiary of Thermo Fisher Scientific 4481 Campus drive Kalamazoo MI 49008 USA

This instrument conforms to the essential requirements of:  

IVD Directive 98/79/EC

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Thermo Fisher Scientific Issue 2.0 – 2013

Important Note Regarding Instrument Variants This document is designed for use with the Thermo Scientific HM525 NX cryostat. Only two variants UVC and none UVC are available on this instrument and the instructions contained in this manual are not specific to any one variant. Thus some features described may not be available on your instrument.

 

 Service Manual Cryostat HM525 NX Cat.No. 391471

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Thermo Fisher Scientific Issue 2.0 – 2013

Welcome This Service Manual is a guide for troubleshooting, field repair and maintenance of the Cryostat HM525 NX.

This Service Manual should be used along with the User Guide (cat. no. 388159) of the Cryostat HM525 NX.

It is important that the service technician reads and understands the User Guide before using this Service Manual.

Errors and omissions excepted. Subject to amendment.

English (printed version): 388159 Print Date: 19/08/2013 Note

This Thermo Scientific product is covered by a guarantee against defects in material or workmanship. Any parts which may fail during the guarantee period will be repaired or replaced free of charge. No further liability shall be accepted. Unauthorized repair or alterations by third parties shall void the guarantee.

 

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Thermo Fisher Scientific Issue 2.0 – 2013

Table of Contents 1 Safety Information

7

1-1

Symbols

7

1-2

Safety Information

8

1-3

General Safety

8

1-4

Chemical Safety

9

1-5

Environment

9

1-6

Warranty Statement

10

2 Introduction to the CryoStat HM525 NX

11

2-1

Intended Use

11

2-2

Instrument Features

11

3 Technical Specifications

12

3-1

Mechanical Specifications

12

3-2

Electrical Specifications

12

3-3

Cooling Specifications

12

3-4

Environmental Specifications

12

3-5

Microtome and Sectioning Specifications

13

3-6

Default Settings

14

4 Maintenance Methods

15

4-1

Dismantling the Casing

16

4-1-1

Remove the Rear Panel

16

4-1-2

Open the Top Cover

17

4-1-3

Remove the Handwheel

18

4-1-4

Remove the middle Part

19

4-1-5

Remove the front panel

20

4-1-6

Remove the Foot panel

21

4-1-7

Replacement of Electronic Components

22

Condensing Unit

24

4-2-1

Replace the complete Condensing Unit

24

4-2-2

Replace the Cooling fan

27

4-2-3

Replace the Starting Relay

28

4-2-3-1

Wiring of the Compressor

29

4-3

Heated Window

30

4-4

Evaporator Heater Mat & Sensor

31

4-2

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Thermo Fisher Scientific Issue 2.0 – 2013

4-5

Electronic Control Unit (ECU)

33

4-6

C-Touch Panel

34

4-7

Cyrobar Peltier, Blade Holder and Chamber Temp Sensor

35

4-8

Microtome

36

4-9

Installation and Removal of the Cross Roller Bearings

37

4-10

Installation and Removal of the Spindle, Cylinder and Motor

39

4-11

Installation and Removal of the Link Block

41

4-12

Microtome Home Position Switch Adjustment

42

4-13

UV-Light (optional)

43

5 Analysis and Troubleshooting

44

5-1

Enter the Service Menu

44

5-2

Overview of the Service Menu

46

5-2-1

Selftest Final Check

46

5-2-2

Maintenance

51

5-2-3

Inputs

56

5-2-4

Outputs

57

5-2-5

Operating Hours

58

5-2-6

Software Update

60

5-2-7

Update C-Touch Software

61

5-2-8

Update C-ECU Software

63

5-2-9

Factory Settings

64

5-2-11

Reset Errors

67

5-2-12

Error Codes

68

5-2-13

Troubleshooting Trees

69

6 Service Assemblies and Spare Parts

78

Appendix A – Cryostat HM525 NX Service Levels

80

Appendix B – PM Procedure

81

Appendix C – Instrument and Service Module Schematics

82

Appendix C-2 – C-ECU

83

Appendix C-3 – Microtome and Motor

84

Appendix C-4 – Condensing Unit

85

Appendix D – Wiring Diagram

86

Appendix E – Schematic of the Refrigerating System

87

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Thermo Fisher Scientific Issue 2.0 – 2013

1

Safety Information

1-1 Symbols The following symbols and conventions may be used throughout this document and on the instrument:    This symbol is used on the equipment, or in a document, to indicate that instructions must be followed for safe and correct operation. If this symbol appears on the instrument, always refer to the operator guide.

 

 

   This symbol is used on the equipment, or in a document, to indicate that there may be a biohazard associated with the instrument. Always act with common sense and be aware of the samples used. Take suitable precautions.    This symbol indicates that a surface is hot. If this symbol appears on the instrument, always refer to the operator guide. Take suitable precautions.

 

   This symbol indicates that a surface is cold. If this symbol appears on the instrument, always refer to the operator guide. Take suitable precautions.  

   This symbol is show in the manual and on the instrument to indicate that hand injuries are possible due to moving parts. Take suitable precautions.  

   This symbol is shown in the manual to indicate the risk of injury caused by sharp microtome blades and knifes. Take suitable precautions.  

 

   This symbol is used on the equipment, or in a document, to indicate that harmful chemicals are used. Refer to the Material Safety Data Sheets for the chemicals used. Always act with common sense and be aware of local laboratory procedures. Take suitable precautions.

Note

Notes give more information about a job or instruction but do not form part of the instructions. A WARNING IS GIVEN IN THE DOCUMENT IF THERE IS A DANGER OF PERSONAL INJURY OR DAMAGE TO SAMPLES OR EQUIPMENT.

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Thermo Fisher Scientific Issue 2.0 – 2013

1-2 Safety Information Thermo Fisher Scientific instruments are designed for convenient and reliable service; however, improper use or handling by a user may damage the instrument, or cause a hazard to health. The instrument must not be used in a manner not specified by Thermo Fisher Scientific. Correct maintenance procedures are essential for consistent performance. It is recommended that users secure a maintenance contract with our service department. Any problems and queries should be referred to your Thermo Fisher Scientific service department.

   The following sections contain important information for the safe setup and use of the instrument, and should be read and understood by the user before using the instrument.

1-3 General Safety This instrument, as supplied, conforms to IEC 61010-1 and IEC 61010-2-101; however, the addition of chemicals introduces potential hazards. Good Laboratory Practice must be employed and consideration must be given to the potential for hazard when dealing with these chemicals.

 

 

Do not use the instrument in close proximity to strong electromagnetic radiation, as these may interfere with the proper operation. The electromagnetic environment should be evaluated prior to operation of the device. Good Laboratory Practice must be used when handling tissue samples to prevent cross contamination and infection. The user should complete a risk assessment to determine any potential hazards related to tissue handling. 

Do not introduce any source of ignition into, or near, the instrument once it has been loaded with reagents.



Do not remove any panels or access covers, unless specifically instructed to do so. The instrument does not have any user serviceable parts. Potentially lethal voltages are present inside the instrument.



The instrument must be properly connected to a good earth (ground) via the Mains input supply and positioned such that it is possible to interrupt the Mains supply at the source by removing the plug from the socket.

  



Use only factory approved accessories or replacement parts within the instrument.

  



Only use reagents recommended in the operator guide.

 

  

  

 

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Thermo Fisher Scientific Issue 2.0 – 2013

1-4 Chemical Safety The introduction of chemicals creates potential hazards. Thermo Fisher Scientific has adopted the following position with regard to the subject of volatile chemicals used in laboratories:



Customers using non-specified chemicals in the instrument, do so at their own risk.



All chemicals recommended by Thermo Fisher Scientific have auto-ignition temperatures considerably above any surface temperatures that can be reached during a single fault failure on the instrument.

  



The instrument contains no source of ignition in any areas of the instrument where chemicals are stored, or likely to leak into, in a single fault condition.

  



The operator is fully aware of the contents of the specification documents detailing the properties of the chemicals they are using.

  



The operator has carried out any legally required assessment of chemicals used and is using Good Laboratory Practice.

  



Some chemicals which may be used during operation are flammable - do not sue sources of ignition in the vicinity of the instrument when it is loaded with reagents.



Harmful chemical vapours such as Xylene or Toluene may be emitted during the normal operation of some instruments and the operator should be aware of suitable precautions and safety measures.

  

 

1-5 Environment This product is required to comply with the European Union's Waste Electrical and Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol:

  Thermo Fisher Scientific has contracted with one or more recycling / disposal companies in each EU Member State, and this product should be disposed of or recycled through them. Further information on Thermo Fisher Scientific’s compliance with these Directives, the recyclers in your country, and information on Thermo Fisher Scientific products which may assist the detection of substances subject to the RoHS Directive are available at: www.thermo.com/WEEERoHS 

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Thermo Fisher Scientific Issue 2.0 – 2013

1-6 Warranty Statement We at Thermo Fisher Scientific are proud of our quality, reliability and of our after-sales service. We continuously strive to improve our service to our customers. Please ask you distributor or Thermo Fisher Scientific representative about Service Contracts which can keep your purchase in peak condition for many years to come. Warranty provisions necessarily vary to comply with differences in national and regional legislation. Specific details can be found in the delivery documentation or from your dealer or representative. Please note that your warranty may be invalidated if: 

This instrument is modified in any way.



Accessories and reagents which have not been approved by Thermo Fisher Scientific are used.



The instrument is not operated or maintained in accordance with the instructions in the Operator Guide.

 

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Thermo Fisher Scientific Issue 2.0 – 2013

2

Introduction

Introduction to the Cryostat HM525 NX 2-1 Intended Use The Thermo Scientific HM525 NX is a high-performance cryostat designed to prepare tissue sections of frozen samples in medical, pharmaceutical and research laboratories. Only trained laboratory personnel may operate the HM525 NX. The instrument may only be operated within the scope of its intended use as described above and as per the instructions given in this manual. Any other use of this instrument is considered as improper operation.

2-2 Instrument Features The HM525 NX is a precision-engineered, ergonomically-designed, high-performance cryostat in modular design with a stainless steel cooling chamber. Some of its features are: 

Colour touch-screen user interface.



A Lighted window for easy positioning.



Intricate freezing device down to max. -55°C via the cryobar



Automatic or manual defrost



Stainless Steel cooling chamber



Chamber temperature regulation variable from +5°C down to -35°C



Additional knee room.

   

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Thermo Fisher Scientific Issue 2.0 – 2013

3

Technical Specifications

3-1 Mechanical Specifications Depth Width Height Range Maximum Weight

760 mm (29.9 in) 640 mm (25.19 in) w/o handwheel 1150 mm ( 45.3 in) 143 kg (315.2 lb)

3-2 Electrical Specifications Power Requirements

BTU

Dedicated line recommended. 100V, 50Hz, 7A, +/-10%, 110-120V, 60Hz, 7A, +/-10% 220-240V, 50/60Hz, 4A, +/-10% 1020BTU/hour

3-3 Cooling Specifications Cryobar Cooling (Peltier Element) Defrosting Options

Max. - 55°C (-67°F) Programmed / Immediate

3-4 Environmental Specifications For Indoor Use Only Temperature (Operating Limits)

+5°C to +35°C (+41°F to +95°F) (at a max. Relative humidity of 60%)

Temperature (Recommended Operation)

+15°C to +30°C (+59°F to +86°F) Note: Performance may deteriorate when operated outside of this range.

Temperature (Transport and Storage)

-20°C to +50°C (-4°F to 122°F)

Relative Humidity

Max. 60% RH up to 35°C

Altitude

Up to 2000 m (6,500 ft)

Pollution Degree

2

Over Voltage Category

II

Floor Loading Requirements

230 kg/m² (507 lb/ft²)

Safety Class

I

Sound Emission

45 dB (A) - measured with 1m distance to the instrument.

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Refrigerant

R404a, filling amount: 100...120V 175g 220...240V 175g

3-5 Microtome and Sectioning Specifications Section Thickness Range

1 – 500 µm

Fine Section Thickness Range

1 – 100 µm 1 – 10 µm in 1 µm steps

Fine Section Thickness Resolution

10 µm – 20 µm in 2 µm steps 20 µm – 50 µm in 5 µm steps 50 µm – 100 µm in 10 µm step

Trimming Thickness Range

5 – 500 µm 5 – 30 µm in 5 µm steps 30 µm – 100 µm in 10 µm steps

Trimming Thickness Resolution 100 µm – 200 µm in 20 µm steps 200 µm – 500 µm in 50 µm steps Specimen Retraction (Return Travel)

20 µm

Vertical Specimen Stroke

64 mm

Horizontal Specimen Movement

28 mm

Specimen Approach

Automated via arrow buttons

Chuck Size

30, 40 mm (additional special sizes are available)

Specimen Orientation

x - and y - axes universal 8° with zero positioning; z - axis up to 360°

Coarse Feed

Motorized

Cutting Drive

Manual via handwheel

Handwheel Lock

Mechanical

Clearance Angle Adjustment

Disposable blade carrier 8- 16°

 

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Thermo Fisher Scientific Issue 2.0 – 2013

3-6 Default Settings Counter Mode

C=0

Retraction

ON

Language

English

Specimen head

-20°C

Fine

0.5 µm

Trim

5 µm

Chamber Light

100%

Screen Brightness

100%

Stepper Motor Initialization

Yes

Cryobar

OFF

Disinfection, Cycle (Automatic)

ON (03:00 Night)

Defrost Cycle (Automatic)

ON (00:00 Night)

Section Counter

0

Section Sum

0

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Thermo Fisher Scientific Issue 2.0 – 2013

4

Maintenance Methods ELECTRICAL SAFETY, DISCONNECT THE CRYOSTAR FROM THE MAINS SUPPLY BEFORE CARRYING OUT ANY OF THESE PROCEDURES. MECHANICAL SAFETY, THE CRYOSTAR CONTAINS MOVING PARTS; TAKE ALL NECESSARY PRECAUTIONS BEFORE CARRYING OUT ANY OF THESE PROCEDURES. MECHANICAL SAFETY, REMOVE ALL BLADES OR KNIVES BEFORE WORKING IN THE CHAMBER OR AROUND THE MICROTOME. BIOHAZARD, ENSURE THAT THE CRYO CHAMBER HAS BEEN DECONTAMINATED BEFORE CARRYING OUT ANY PROCEDURE THAT REQUIRES WORKING INSIDE THE CRYO CHAMBER.

POTENTIALLY HAZARDOUS SUBSTANCES, THE CRYOSTAR CONTAINS POTENTIALLY HAZARDOUS SUBSTANCES (E. G. DISINFECTANT). TAKE ALL NECESSARY PRECAUTIONS WHEN WORKING WITH THESE SUBSTANCES.

MICROTOME MAY BE COLD TO HANDLE AFTER POWER SHUT OFF, HANDLE WITH PROTECTIVE GLOVES

 

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Thermo Fisher Scientific Issue 2.0 – 2013

4-1 Dismantling the Casing Note

All metal casing parts are secured with a grounding connection, to remove the casing parts you have to disconnect this connection. Please ensure that you reconnect this connection when you reassemble the casing parts again.

4‐1‐1 Remove the Rear Panel Tools required:  Phillips Screw Driver Parts required:  None Procedure: Use a Phillips Screw Driver to remove the 10 Phillips screws that secure the rear panel. Carefully lift the rear panel off and place it to the side.

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4‐1‐2 Open the Top Cover Tools required:  Screw Driver Parts required:  None Procedure: Open the heated window. Use a Screw Driver to remove the two screws from inside the front of the top cover. Lift off the top cover. The cover has a fixation on the side, if you lift it off to the end it will stay in this position.

 

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4‐1‐3 Remove the Handwheel Tools required:  6 mm Allen Key  Screw Driver Parts required:  404410 Cover disk Procedure: THE HANDWHEEL IS HEAVY. TAKE CARE THAT THE HANDWHEEL IS SECURED BY ACTIVATING THE MECHANICAL BRAKE WHILE UNSCREWING IT.

Use a screw driver to carefully remove the cap from the middle of the handwheel. Use the 6 mm Allen Key to remove the screw holding the handwheel. Note

The handwheel handle should be in the DOWN position before removing.

Be careful of the spring and the bolt of the mechanical brake mechanism when you slide the handwheel down. To reassemble the handwheel again, position it in the high position and the specimen head also. Now you can see the bolt through the hole in the handwheel. Push them together and fix the handwheel with the Allen screw.  

Service Manual Cryostat HM525 NX   Cat.No. 391471

 

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4‐1‐4 Remove the middle part Tools required:  3 mm Allen Key Parts required:  None Procedure: Open the top cover – see 4-1-2 Open the top cover. Use the 3 mm Allen Key to remove the two screws that secure the middle part. Carefully lift the part off and place it to the side.

 

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  4‐1‐5 Remove the front panel Tools required:  3 mm Allen Key Parts required:  None Procedure: Open the top cover – see 4-1-2 open the top cover. Use the 3 mm Allen Key to remove the three screws at the front and lift off the front panel and place it to the side.

     

Service Manual Cryostat HM525 NX   Cat.No. 391471

 

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  4‐1‐6 Remove the Foot panel Tools required:  3 mm Allen Key  8 mm Wrench Parts required:  None Procedure: Remove the rear panel – see 4-1-1 remove the rear panel. Open the top cover – see 4-1-2 open the top cover. Remove the front panel – see 4-1-5 remove the front panel. Use the 3 mm Allen Key to remove the two screws at the right side and the one on the bottom. Use the 8 mm wrench to undo the nuts on the other side of the foot panel.             

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  4‐1‐7 Replacement of Electronic Components Following replacement of any electronic component (see list below), carry out the following steps to  check the functionality of Cryostat.  ‐ ‐ ‐ ‐ ‐

Turn OFF the Cryostat  Check the wiring connections between the new component and the C‐ECU, according to the  wiring diagram    Insert the Thermo USB‐stick.  Turn ON the Cryostat; the Login‐Screen appears.  Press LOGIN and enter the Service password.  YOU MUST CARRY OUT THE FOLLOWING STEPS TO TEST ALL   ELEMENTS.

‐ ‐

‐ ‐

‐ ‐ ‐ ‐ ‐ ‐ ‐

‐ ‐

Press the „SOFTWARE UPDATE“‐button to check the on‐board software versions. If required  update the appropriate software to the most‐ current version.  Press the “INPUTS”‐button. The Service‐Inputs screen appears. Check the indicated switches.  The switches light barrier top reversal point and Microtome home position are normally on,  as indicated by a yellow light. The remaining switches are normally off and no yellow light  shows. The test is successful when the indicator changes color.  Press the „OUTPUT“‐button. The service‐Output Screen appears.  Press the “ON”‐button for chamber light. Press the “INPUTS”‐button and review the color  status of the relevant components. Green = OK, Red = current very high, yellow = not  connected or current very low, White = not ON or not connected.  For other components press the relevant button (e.g. Compressor) and follow the above  procedure.  To resolve/repair any red‐ or yellow – colored value follow the procedure in the relevant  troubleshooting tree.   Create a service protocol to log all repairs completed.  Perform the fully automated test but abort the test on upon reaching the COOLING A step.  Now perform the Semi automated test to ensure full system functionality.  Check the Service log‐file to ensure that all error messages have been cleared.  When all “INPUTS“ and “OUTPUTS“ are checked press the “RETURN”‐button to display the  main screen. The chamber light is now illuminated and the Specimen head advances to the  home position.  Press the “DATA LOG”‐button on the menu‐settings screen. Ensure that all error messages  have been cleared.  Remove the USB‐Stick and restart the Cryostat. 

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  Note

In case of replacement of any electronic component, always perform the above procedure. List of Electronic Components  ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐

Replace defrost heater, defrost sensor, Cryobar Peltier  Replace Compressor, Fan, chamber temp sensor and ambient temp. sensor  Replace C‐ECU box and Cables  Replace Power Supply (24V)  Replace heating window , chamber light and rear contact switch  Replace Electronic Connector panel (line filter, main switch, USB etc.)  Replace Touch screen/TFT  Replace fuses  Replace C‐ECU Fan   

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4-2 Condensing Unit 4‐2‐1 Replace the complete Condensing Unit Tools required:     

5 mm Allen Key 22 mm Wrench 24 mm Wrench 10 mm Wrench Evacuation Equipment

Parts required:  435380 (115V) / 435370 (230V) Condensing Unit assembly  261690 Armaflex Insulation tape Procedure: Power down unit and unplug it. Remove the rear panel of the instrument – see 4-1-1 Dismantle the Rear Panel. Use the Allen Key to unscrew the two screws on the bottom left hand side and remove the nut in the middle with the 10 mm wrench. Lift up the whole condensing unit a little bit and move it to the left side approximately 10 cm (4 inches).  

Service Manual Cryostat HM525 NX   Cat.No. 391471

 

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Thermo Fisher Scientific Issue 2.0 – 2013

  Remove the Armaflex insulation from the tubing around the two quick couplings. Use two wrenches size 22/24 mm to disconnect the two quick couplings.

Disconnect the wiring from the condensing unit and remove it. Insert the new condensing unit assembly into the instrument in reverse order. THIS AND ALL OTHER WORK MUST ONLY BE CARRIED OUT BY AUTHORIZED REFRIGERATION TECHNICIANS WHO HAVE THE CORRESPONDING TOOLS AND DEVICES AT THEIR DISPOSAL!

Use a refrigeration evacuation and filling system (i.e. ATP Promax Minimax®) to remove all old refrigerants from the system for recycling.

Note

Dispose of the refrigerant in accordance with your national rules and regulations.  Service Manual Cryostat HM525 NX Cat.No. 391471

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Evacuate the system and refill it with refrigerant (R404A – 175g +/- 2g). Note

The evacuation process takes a minimum of 45 minutes. Turn on the instrument and plug in the service USB-Stick. Enter the Service Menu and execute the condensing unit test procedure – see chapter 5, Test Procedures.  

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  4‐2‐2 Replace the Cooling Fan Tools required:  5mm Allen Key  Ratchet  10 mm socket  250 mm Extension Parts required:  525080 (115V) / 525090 (230V) Cooling Fan assembly Procedure: Power down unit and unplug it. Remove the rear panel of the instrument – see 4-1-1 Dismantle the Rear Panel. Disconnect the fan from the connector box at the compressor. Unscrew the two nuts (1) from the fan bracket mounted to the frame and remove the fan assembly.

Insert the new fan assembly and tighten the two nuts. Reconnect the fan with the connector box. Fix the condensing unit assembly with the two screws to the frame. Reassemble the rear panel of the instrument.  

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  4‐2‐3 Replace the Starting Relay Tools required:  5mm Allen Key  Phillips Screwdriver Parts required:  362810 (115V)/ 362820 (230V) Starting Relay Procedure: Power down unit and unplug it. Remove the rear panel of the instrument – see 4-1-1 Dismantle the Rear Panel. Remove the cover from the connector box, which is attached to the compressor, to access the starting relay.

Remove the wiring from the relay. Remove the faulty relay and insert the new one Reconnect the relay. Reinstall the cover of the connector box. Reassemble the rear panel of the instrument.  

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4‐2‐3‐1 Wiring of the Compressor

 

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4-3 Heated Window Tools required:  3 mm Allen Key  Wire Cutter Parts required:  625940 Heated Window assembly Procedure: Open the top cover – see 4-1-2 Open the Top Cover. Disconnect the heated window from the socket and cut the wire tie. Remove the heated window and replace it with a new one if required.

Note

Ensure that the window moves freely and that there is no excessive side to side movement. Use the long guide rail to set a gap of 1 mm, side to side, between the heated window and the rail. Reconnect the heated window and close the top cover.

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4-4 Evaporator Heater Mat & Sensor THERE MAY BE SHARP EDGES IN THIS AREA; CARE SHOULD BE TAKEN WHEN WORKING NEAR THE EVAPORATOR.

Tools required:  2.5 mm Allen Key  3 mm Allen Key  Long Nose Pliers (or similar) Parts required:  626470 Heater Mat  320900 Evaporator Temp. Sensor  2 Cable ties Procedure: Remove the rear panel – see 4-1-1 Dismantle the Rear Panel. Open the top cover – see 4-1-2 Open the Top Cover. Remove the heated window. Remove all screws from the evaporator cover. Remove the socket for the heated window connector. Remove the white screen from the front of the evaporator. Heater Mat

Disconnect the heater mat from the C-ECU at the connector X15-A, 1+2. Use long nose pliers (or similar) to ease the heater mat forward from underneath the evaporator and out of the instrument. Push the new heater mat under the evaporator. Reconnect the heater mat. Note

The heater mat is not fixed in position. After replace ensure bottom of the heater mat is uniformly touching along the bottom of the metal tray, this will ensure equal heating  

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  Evaporator Temp Sensor

Disconnect the electrical connection to the temp sensor at X15-A, 3+4.

2

1

1

Heater Mat

626470

2

Evaporator Temp. Sensor

320900

Ease the insulation upwards to gain access to the temp sensor. BE CAREFUL NOT TO DAMAGE THE INSULATION.

Cut the cable ties that are holding the temp sensor in position and remove it. Use new cable ties to hold the new temp sensor in position. Connect the sensor. Replace the white screen in front of the evaporator. Replace the insulation. Refit the evaporator cover, the heated window and the top cover. Note

Check the movement of the heated window and adjust it if required.   Service Manual Cryostat HM525 NX   Cat.No. 391471

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4-5 Electronic Control Unit (C-ECU) Tools required:  5 mm Allen Key Parts required:  435590 Electronic Control Unit assembly Note

The C-ECU is protected by a metal cover and is replaced as a complete assembly.

Procedure: Remove the rear panel– see 4-1-1 Dismantle the Rear Panel. Disconnect all plugs from the C-ECU assembly. Note

Note the positions for reconnection. Use the 5 mm Allen Key to remove the four M6 Allen screws that secure the C-ECU and remove it. Replace the C-ECU assembly as required and secure it to the chassis using the four M6 Allen screws (as above). Reconnect all of the plugs. Replace the rear panel.  

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4-6 C-Touch Panel Tools required:  2.5 mm Allan Key Parts required:  436210 Touch Screen Procedure: Remove the two screws from the rear of the touch panel housing and move the touch panel forward. Disconnect the electrical connections to the touch panel and remove it from the top cover assembly. Remove the SD-Card from the old Touch Screen Insert it into the new Touch Screen Replace the touch panel as required. Reconnect the electrical connections. Fit the touch panel back into the housing ensuring that the outer gasket is retained in position. Refit the two screws that secure the touch panel screen to the housing. Restore Data: The SD-Card is a backup of the data from the Touch Screen. Information like operating hours, serial number and quality test are saved on this card. To keep this data in case of a defective Touch Screen, insert the card from the old Touch Screen in the new one. After restarting the instrument with the new Touch Screen the window below will appear:

Please push this button to copy the files from the SD-Card to the new Touch Screen.

Note

If you don’t perform this copy procedure the Touch Screen will overwrite the SD-Card and all old information on the card will be deleted.   Service Manual Cryostat HM525 NX   Cat.No. 391471

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4-7 Cryobar Peltier, Blade Holder and Chamber Temp Sensor BIO-HAZARD, MAKE SURE THAT THE CRYO CHAMBER HAS BEEN DECONTAMINATED BEFORE WORKING INSIDE THE CHAMBER. REMOVE ALL KNIVES / BLADES BEFORE WORKING IN THE CHAMBER.

Cryobar Peltier Tools required:  2 mm Allen Key Parts required:  336140 Peltier Element Procedure: Use the Allen Key to remove the fast freezing station from the cryobar. Disconnect the electrical connection to the cryobar at the plug. Remove the peltier from the cryobar assembly.

Clean out any residual adhesive and thermal compound. Insert the cable from the new peltier through the access hole in the cryobar assembly. Attach the new peltier to the cryobar assembly using the adhesive thermal compound. Replace the fast freezing station.

Note

Don’t over tighten the screw because it might crack or damage the peltier  

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4-8 Microtome BIO-HAZARD, MAKE SURE THAT THE CRYO CHAMBER HAS BEEN DECONTAMINATED BEFORE WORKING INSIDE THE CHAMBER. REMOVE ALL KNIVES / BLADES BEFORE WORKING IN THE CHAMBER. ENSURE MICROTOME IS DRY AND SAFE TO HANDLE DUE TO COLD TEMPERTURE

Note

In order to remove the Microtome it is first necessary to remove the Waste Tray, Right Chamber Cover, Left Chamber Cover and the Blade Holder. Disconnect the electrical connector on the left side of the microtome and remove the temperature sensor. Tools required  5 mm Allen Key Parts required  None Procedure Open the top cover see 4-1-2, remove the heated window see 4-3, use the 5 mm Allen Key to remove the M5 screw that secure the complete Microtome in the chamber.

Note

Turn the hand wheel into the lower position, i.e. the Specimen Head is also at the lower reversal point. Slightly move the Microtome to the left approximately 2cm until the clutch on the right side of the Microtome is free. Remove it carefully from the chamber. To reinstall the Microtome follow the steps in reverse order. Service Manual Cryostat HM525 NX   Cat.No. 391471

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4-9 Installation and Removal of the Cross Roller Bearings Tools required    

2 mm Allen Key 2.5 mm Allen Key 3 mm Allen Key Torque Key

Parts required  518330 Cross Roller Bearings Procedure Removing: Remove the Microtome from the chamber see 4-8 and place it on a bench. Loosen the Allen screws which fix the cover on the Microtome and lift it off.

To remove the specimen head undo the 5 mm Allen Key screw in the centre of the head. To get access to the cross roller bearings remove the 4 Allen key screws that fixes the plastic plate

Remove the right roller bar. Remove the two cross roller bars from the mono block. Remove the cross roller bar from the left.   Service Manual Cryostat HM525 NX   Cat.No. 391471

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  Installing:

E

D

C

B

3 2 A

1 2 3

Assemble all cross roller bars as disassembled, don’t tighten the screws. Place the screws slightly in place so that the screw heads are touching the bottom of the cross roller bar and the cross roller bars touch the base. Make sure the cross roller bearings are pushed all the way to the right. Push the row (B) against the wall and tighten it. Use the following sequence of fastening: ○ 1 ○ 2 ○ 2 ○ 3 ○ 3 Set a torque of 9Ncm with the torque key on the adjustment screws (A) on the left side. Fasten the screws beginning at the right bar (C). Repeat this step for all cross roller bars from C to E. Reassemble the specimen head and the blade holder. Use the fastening sequence for all rows.

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4-10 Installation and Removal of the Spindle, Cylinder and Motor Tools required      

362200 Adjusting Face Spanner 362110 Key for Micrometer Spindle 362090 Key for Micrometer Nut 2 mm Allen Key 3 mm Allen Key Pliers

Parts required  550220 Spindle, Nut and Bearings Procedure Remove the microtome – see 4-8 Microtome. Remove the specimen head- see 4-9. First loosen the Multi Function Cable. Next, dismount the Microtome. Loosen the Flexible Coupling from the end of the Micrometer Assy. by unscrewing the 2mm screw through one of the access holes on the Distance Sleeve. Now you can remove the 4 3mm bolts that fix the Stepper Motor to the Distance Sleeve.

 

To remove the Micrometer mechanism from the Cylinder proceed as follows: Use the Adjusting Face Spanner (cat. no. 362200) to hold the Threaded Ring in place and at the same time use the Key for the Micrometer Spindle (cat. no. 362110) to turn the Spindle from the front in a counter clockwise direction. Now you are able to remove the Equalizing Discs, the axial bearing discs, the axial needle wheel and the axial disc from the micrometer spindle. Next use the Offset Key for the Micrometer Nut (cat. no. 362090) to remove the Spindle and Nut Complete. To further disassemble the micrometer nut and the Counter nut, unscrew them from micrometer spindle.

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Service Manual Cryostat HM525 NX   Cat.No. 391471

 

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4-11 Installation and Removal of the Link Block Tools required  Allen Key 2.5 mm  Allen Key 4 mm Parts required  520110 Link Block Service Set Procedure Remove the Microtome – see 4-8 – take off the metal cover that you have access to the shaft with bearing. Remove the 4 Allen key screws and carefully pull out the shaft with bearings.

Note

Before installing a new link block, clean all bearing surfaces. Remove old grease and lubricate the bearings surfaces as well as the cylinder pin with isoFlex grease (cat.no 350140). Check the link block for smooth movement on the guide rail. If the movement on the guide rail is not smooth, carefully sand the link block with sandpaper (1000 grade). Note

Be careful! Do not over-sand. Besides the nominal size of 15.00 mm, link blocks with diverging tolerances of 15.02 mm or 14.98 mm are available for special needs: Standard size: Oversize: Undersize: Service Manual Cryostat HM525 NX   Cat.No. 391471

15.00 mm, cat. no. 421050 15.02 mm, cat. no. 421060 14.98 mm, cat. no. 420630 Page 41

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4-12 Microtome home position switch adjustment Tools required  3 mm Allen Key  Screw driver  614150 Checking line for Microtome Parts required  none Procedure Move the Specimen head to its end position by pushing the button on the display (furthest position away from the blade holder). Switch off the instrument. Remove the Microtome see 4-8, remove the specimen head see 4-9 that you have access to the spindle. Use the micrometer spindle tool and rotate counter clockwise until you reach the mechanical end position. From this point rotate the spindle in the opposite direction by 3-5 revolutions. Check if the home position light barrier is still engaged. Flag (2) shouldn’t be engaging light barrier board if so then adjust the flag with the screws (3).

Note:

Ensure that the Flag is absolutely parallel to the light barrier to have a clear switching point

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4-13 UV-Light (optional) Tools required  Screw driver Parts required  346230 UV-lamp Procedure Open the Top cover see, 4-1-2 open the top cover. Turn the heated window over so that you have access to the UV lamp. Disconnect the UV lamp and put the new one in.

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5

Analysis and Troubleshooting

This chapter supports service technicians in self-contained searches for malfunctions, their corresponding analysis and elimination. Because of the technical and mechanical complexity of the CryoStat HM525 NX, this chapter is divided into the different modules. This structure provides the reader with a more efficient overview. The described failures and malfunctions might occur during the daily use of the CryoStaT HM525 NX.

Note!

If this information is not sufficient to fix a malfunction or failure, please contact the Factory Service Support Team immediately.

5-1 Enter the Service Menu The Service Menu provides the software tools and test procedures for the service technician. To enter the Service Menu: Press the “Menu” button on the Main screen.

The Menu screen appears.

Press the “Service” button.

 

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  The Password screen appears.

Note

The password to enter the Service menu is walldorf. All in small letters. Press the “Selection” button once to highlight the small letter “a”.

The keyboard will show small letters. Use the keyboard to type in the password.

Press the “OK” button to enter the service menu.

Press the “Back” button to return to the Main screen.

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5-2 Overview of the Service Menu The buttons on this screen show the available Service options.

Press the “Back” button to return to the Main screen

5‐2‐1 Selftest Final Check Enter the Service menu as described above. Press the “Selftest Final Check” button.

The “Service – Selftest Final Check” screen will appear.

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To perform the Fully Automated Test (FAT): Note

The Fully Automated Tests checks all electromechanical functions of the CryoStaT. An on-screen status report is provided at the conclusion of the Fully Automatic Test. Press the “Fully Automated Test” button. The “Service - Selftest Final Check - Fully Automated Test” screen appears.

Press the “Start” button to start the Fully Automated Test.

The “Service - Selftest Final Check - Fully Automated test” screen appears.

Note

The ‘‘Back’’ button is deactivated during this test routine. Press the “ABORT” button to stop the test routine.

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Press the “Back” button to return to the Service – Self Test Final Check screen. Press the “Back button to return to the Service menu. Notes

The elapsed time since the „START‘‘-button was activated is shown on the top left of the screen. Errors are listed in the uppermost grey field. The middle grey field is the status bar. The length of the yellow bar reflects the progress of the test. The lowest grey field indicates the test routine currently in progress. An on-screen status report is provided at the conclusion of the ‘‘Fully-Automated Test’’.   The Fully Automated Test checks all of the following components:  

Components 

1. 2. 3. 4. 5. 6. 7. 8. 9. 10.

Chamber lighting LED (front) Fan (C-ECU) Window Heater Defrost Heater Peltier element (cryobar) Home Switch Microtome & Stepper Motor Compressor temperature sensor Ambient temperature sensor Defrost temperature sensor General cooling test (approx 9 hours)

The Routine during fully Automated Test: Cooling A Cooling B Cooling C Defrost Test Finished Reboot and Start Normal GUI

3 min to 6 hours 1 hour 1 hour Max. 60 minutes

-35°C/6h Chamber Temperature -25°C Chamber Temperature -20°C Chamber Temperature

 

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  Press the “Semi Automated Test” button.

The “Service - Selftest Final Check - Semi Automated Test” screen appears.

Note

The upper gray field is the status bar. The length of the yellow bar reflects the progress of the current test. The lower gray field identifies the test in progress. On completion of the test, the next test is identified on the lower grey field. Press SKIP/ FAIL/ OK to proceed. Press the “OK” button to start the Semi Automated Test.

Press the “SKIP” button to skip the current test and to move on to the next

Press the “FAIL” button to record a failed test in the error log and to display the next available test.

Press the “Back” button to return to the previous menu.

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  To perform the Semi Automated Test (SAT): The “Semi Automated Test” provides the capability to test selectable, individual system functions. Neither temperature tests nor checking of temperature sensors is included in the “Semi Automated Test”. In the “Semi Automated Test” the following test options are presented sequentially. If the test passes press the “OK” button to display the next available test. If the test fails, press the “FAIL” button to record the failure in the error log and to display the next available test. 1. 2. 3. 4. 5. 6. 7.

Press Trim key to test 10µm ? Press Trim key to test 500µm ? Open Heated Window Close Heated Window Does the Cryobar Temp Reach its Max Temp within 2 Minutes ? Cryobar Cooling Test Timed Out Semi-Automated Test Completed

 

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  5‐2‐2 Maintenance This menu provides access to the following tasks:    

View and export error logs. View and export maintenance logs with time stamps and PM record. Service maintenance protocol. Log-File listing all service events.

To enter the “Service – Maintenance” screen: Press the “Maintenance” button on the Service screen to access the “Service – Maintenance” screen.

The “Service - Maintenance” screen appears.

Logfile: To export the logfiles: Press the “Logfile” button.

 

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  The “Service - Maintenance - Logfile” screen appears.

Press the “Export” button to save the log files to a USB-Stick.

Note

The log files can be subsequently exported to an Excel file. Press the “Back” button to return to the “Service - Maintenance” screen. The following events are automatically saved in the logfile:  Complete listing of error-messages.  Error log information will be saved for a period of one year following FIFO (“first-in, first-out”) and then archived to a SD-memory card.  Log File information is maintained in a temporary file which, when full, automatically archives the information to the SD-memory card.  The log file information also provides the serial number of the cryostat as well as system configuration information in logfile header.  The following information is saved on the SD-memory card: user logs, error logs, PM-protocols, operating settings, instrument serial number and build configuration. Note

To export the user log file in the user Application you must enter the Password ‘‘dataLog’’ with a big ‘‘L’’. When you export this file it will include the Quality Test results, the Maintenance Protocols, the Service and user Log files.

 

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  Maintenance Protocols: This menu provides access to the following tasks and information:     

Information on Service tasks and completion dates. Creating of a maintenance-worklist. Editing according to PM-protocol. Checkbox on every single test, must be timestamp. Space for inclusion of the Field Service Engineer’s name (must be filled in) and for notes (optional).

To access the “Service - Maintenance - Maintenance Protocols” screen: Press the “Maintenance Protocols” button.

The “Service - Maintenance - Maintenance Protocols” appears.

Press on an existing maintenance protocol to review or export it. To create a new maintenance protocol: Press the “New Protocol” button.

 

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  The “Service - Maintenance - Maintenance Protocols - (date)” screen appears.

Notes

Unchecked tasks are marked with a question mark. Checked tasks are marked with ‘‘Done’’.

Press the “Question mark” button to mark the completed task as “Done”. Note

It is mandatory to fill in the name of the Service technician performing maintenance on the instrument. Use the scroll bar on the left side to scroll down to the “Name” field.

Press on the “Name” button to enter your name using the appearing keyboard. Press the “OK” button to save your name and to return to the “Service - Maintenance - Maintenance Protocols - (date)” screen. Press the “Export” button to export the maintenance protocol to the USB-Stick.

 

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Check that the following information is saved to the SD-memory card: user logs, error logs, PM-protocols, operating settings, instrument serial number and build configuration. Press the “Back” button to return to the “Service - Maintenance” screen. Overview of the available Service Maintenance Items: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15.

Condenser Condenser Fan Link Block Knife Carrier Clamps Microtome Guide Bars Microtome Spindle Microtome Advance Movement Object Chuck Clamps Cooling Performance Defrost Function C-Touch Handwheel Zero Position Handwheel Belt Handwheel Brake Function Crossroller Guide Bars

 

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  5‐2‐3 Inputs The “Service - Inputs” screen provides a status report on all system inputs, using the following color highlighting: White: Not connected Green: O.K. Red: Over Limit Yellow: Below threshold value To access the “Service - Inputs” screen: Press the “Inputs” button on the Service screen to access the “Service - Inputs” screen.

The “Service - Inputs” screen appears.

Note

This screen displays inputs as ON/OFF, current (mA) and temperature (°C)

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  5‐2‐4 Outputs This menu is used to test the outputs of the CryoStar.  Test the individual components of the cryostat.  Use the “Inputs” and “Outputs” buttons to toggle between the two screens.  Pressing the “Back” button cancels any changes made in the output screen. To access the “Service - Outputs” screen: Press the “Outputs” button on the Service screen to access the “Service - Outputs” screen.

The “Service - Outputs” screen appears.

This menu is used to:  Check all outputs that can be directly tested.  Check if input value change by pressing the appropriate ON-button.  Check if all values are “OK” by toggling between the “Service - Input” and “Service - Output” screens.  Check if the switching between “Inputs” and “outputs” is working.  Use the scroll-bar on the left to scroll up and down. Press the “Back” button to return to the Service menu.  

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  5‐2‐5 Operating Hours This menu provides access to Operating Hours functions. It provides the following tasks and information:  Tabulates elapsed hours of operating for all electrical subsystems.  Export of the operating hours logfile to a USB-Stick.  The operating hours for all listed subsystems can be reset. Notes

The operating hours log is saved for the life time of the instrument. The operating power meter is automatically saved and provided with the maintenance protocol --see 5-2-2 Maintenance To enter the “Service - Operating Hours” screen: Press the “Operating Hours” button on the Service screen to access the “Service - Operating Hours” screen.

The “Service - Operating Hours” screen appears.

Use the scroll bar on the right side to scroll through the screen. Press the “Reset” button to reset the selected counter.

 

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  A warning message appears.

Press the “OK” button to reset the selected counter. Press the “Abort” button to return to the “Service - Operating Hours” screen without resetting the counter. Press the “Back” button to return to the “Service - Maintenance” screen. Overview Operating Hours of electrical devices: Reset not possible 1. 2. 3. 4. 5. 6. 7. 8. 9. 10.

Unit Compressor Compressor Fan Stepper Motor Chamber LED Light UV-Lamp Power Supply C-Touch Display (Back LEDs operating hours) C-ECU

Reset possible

X X X X X X X X X X

 

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  5‐2‐6 Software Update This menu enables the Service Technician to perform Software updates on the instrument. The following tasks can be performed:  Software updates for C-ECU C-TOUCH Language file updates User Guide & Service Manual updates To enter the “Service - Software Update” screen: Press the “Software Update” button on the Service screen to access the “Service - Software Update” screen.

The “Service - Software Update” screen appears.

Note

The first field on this screen provides important information about the current installed software version. Update performed by a Service Technician: The user GUI file can be updated directly by placing it in the /Service/Update/ path. All other files (user GUI, Language, Service GUI and Service Manual files and folder) must be packed in a zip folder as “ServicePack.zip” and placed on the USB-stick under /Service/Update/, or locally under /CTouch/Service/Update/. Note

The files in ‘‘ServicePack.zip’’ will overwrite the old files.  

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  5‐2‐7 Update C‐Touch Software The user GUI file can be updated directly by placing in the /Service/Update/ path. Insert the USB-Stick in the USB-port on the connections panel.

The “Service - Software Update - C-Touch” screen appears.

Press the “check USB-stick” button.

When the USB-stick is recognised the name of the update file appear on the screen. Press the file to highlight it. Press the “Copy?” button.

Press the “Start C-Touch Update” button.

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  Note

Within a short period the name of the new software version appears on the top left of the screen. Reboot the C-Touch. All other files (user GUI, Language, Service GUI and Service Manual files and folders) must be packed in a zip folder as “ServicePack.zip” and placed on the USB-stick under /Service/Update/. Insert the USB-Stick in the USB-port on the connections panel. The “Service - Software Update - C-Touch” screen appears.

Press the “check USB-stick” button.

When the USB-stick is recognised the name of the update file appears on the screen. Press the file to highlight it. Press the “Copy?” button.

Press the “Start additional Update” button.

Note

Within a short period the name of the new software version appears on the top left of the screen. Reboot the C-Touch.

Press the “check local” button if the files have been saved locally on C-Touch and then update the files.

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  5‐2‐8 Update C‐ECU Software The user C-ECU firmware can be updated directly by placing in the /Service/Update/ path. Insert the USB-Stick in the USB-port on the connections panel and reboot the instrument.

Lock in as Service. Enter the Service menu and press the “Software Update” button on the Service screen to access the “Service - Software Update” screen.

The “Service - Software Update - C-ECU” screen appears.

Press the “check USB-stick” button.

When the USB-stick is recognised the name of the update file appears on the screen. Press the file to highlight it.

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  Press the “Load?” button.

Press the “Start Firmware Update” button.

Notes

The system now goes into the Bootloader Mode. It will take about 2 minutes to update the software. When the update is complete, the identity of the new software version and ‘‘Success’’ appears at the top left of the screen. Restart the instrument.

5‐2‐9 Factory Settings This menu provides information about:  System configuration or build options.  Serial numbers and software versions. To enter the “Service - Factory Settings” menu: Press the “Factory Settings” button on the Service screen.

The “Service - Factory Settings” screen appears.

 

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  System Configuration: Press the “System Configuration” button.

The “Service - Factory Settings - System Configuration” screen appears.

Note

This screen is used to review the system build options for the Cryostat HM 525NX. This screen provides a status report on system configuration, using the following color highlighting: Yellow: This build option is implemented and available for use. White: This build option is available but not implemented.

Note

The selected model is highlighted in yellow. Pressing on the white button UV-Disinfection enables this option in the system software. Press the “Back” button to return to the previous screen. Serial Numbers: This submenu provides information about the serial numbers of the installed electronic boards. Press the “Serial Number” button on the “Service - Factory Settings” screen.

 

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  The “Service - Factory Settings - Serialnumber” screen appears.

Note

Installed electronic boards: C-Touch and C-ECU Press the “Back” button to return to the “Service - Factory Settings” screen. Language Selection: From the Main Menu, press the Language Selection button. The following screen will be shown:

Select the required language and then press the return arrow button to go back to the main screen.  

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  5‐2‐11 Reset Errors Press the “Reset Errors” button on the Service screen to check on the effectiveness of a repair procedure.

Notes

The repair procedure is effective if the error message disappears from the screen and does not return. If the specific error message returns, recheck the original repair or look for another root cause for the problem. To verify and troubleshoot all error codes refer to the ‘‘ErrCodes-V0xx_Thermo.xlsx and Limits.xlsx  

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  5‐2‐12 Error Codes ErrorNo

Name

001-001

Defrost Heating mat error

001-002

Evaporator temperature error

001-003

Defrost cycle interrupted either by User or by power failure. Defrost incomplete

001-004

Defrost time should not overlap with disinfection cycle. Disinfection will begin 2 hours after completion of defrost cycle

001-005

Cryobar Peltier error

001-006

CPU fan error

001-007

Chamber light error

001-008

Defrost sensor error

002-002

Ambient Temperature error

004-001

Stepper home position error

004-007

Window heating error

004-011

Chamber temperatur sensor error

005-001

C-Touch communication error

007-002

Defrost time should not overlap with disinfection cycle. Disinfection will begin 2 hours after completion of defrost cycle

007-003

Disinfection cycle interrupted either by User or by power failure. Disinfection incomplete

007-004

Close window to start disinfection cycle

007-009

Close window to start Disinfection

007-012

Close Window & Start Disinfection cycle once again

007-014

Efficiency of the UV-lamp reduced due to operating hours

008-002

Change UV-lamp

 

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  5‐2‐13 Troubleshooting Trees   Error code

Troubleshooting tree number 

Page

001-001 001-002/001-008 001-005 001-006 001-007 008-002 002-002 004-001 004-007

TT 05 Evaporator Heating TT 06 Evaporator Sensor TT 09 Cryobar Peltier TT 01 CPU Fan TT 03 Chamber Light TT 04 UV-Light TT 07 Ambient Sensor TT 08 Contact Switch Microtome TT 02 Window Heating TT Startup Temerature error

73 74 76 70 72 72 74 75 71 77

   

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6

Service Assemblies and Spare Parts

Module / Description

Part Number

Module: Chassis Top Panel Front Panel Middle Panel Cryo Chamber Lower Cover Cryo Chamber (foot panel) Rear Panel Door Waste Bottle Heated Window Heated Window with UV-light (230V) Heated Window with UV-light (115V) Hand Wheel Assy, with screw and Cover Disk Cover Disk Window OPEN/CLOSE microswitch UV-lamp

443100 419650 419660 419670 419630 419640 261980 625940 625950 625960 534200 404410 322010 346230

Module: Refrigeration Refrigeration Module, 115 V Refrigeration Module, 230 V Fan Assembly Refrigeration Module, 115 V Start Relays Assembly, 115 V Fan Assembly Refrigeration Module, 230 V Start Relays Assembly, 230 V Cryobar Peltier Evaporator Temp Sensor Ambient Temp Sensor Evaporator Heater Mat

435380 435370 525080 362810 525090 362820 336140 320900 320900 626470

Module: Electronic Touch Screen C-ECU, Main PCB Box Power Supply Assy Main Plug (115V) Main Plug (230V) Line Filter USB-Stick Fuse Kit for C-ECU  

Service Manual Cryostat HM525 NX   Cat.No. 391471

436210 435590 630440 329620 321100 265020 612070 626310  

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  Module: Microtome/ Blade Holder Stepper Motor Light Barrier Board Crossroller Bearings Spindle, Nut and Bearings Specimen Head cpl. Link Block Service Set Shaft with bearing Guidance with socket Knife Holder C cpl. Blade Holder EC cpl. Blade Holder EC Upper Part Glas Anti Roll Plate Chamber Temp Sensor Link Block Kit Cryostat Oil Iso Flex Grease

320910 550430 518330 550220 556070 520110 403250 550240 705460 705630 566210 449980 320900 520110 350040 350140

Special Tools: Torque Key Inserts for Torque Key Dial Gauge Dial Gauge Holder Spring scale for micrometer mechanism Torque measurement device for micrometer spindle Key for micrometer spindle Adjusting face spanner Size 2 Key for micrometer nut Checking line for Microtome 2.5m

362060 362290 362070 648010 362080 358170 362110 362200 362090 614150

 

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Appendix A – Cryostat HM525 NX Service Levels Maintenance levels Level I: User maintenance Installation Turning-on Cleaning Installing accessories Level II: Service technician maintenance Routine maintenance Installing accessories Diagnostics Replacement of assemblies and parts Executing service contracts Level III: Factory maintenance Repair of assemblies and parts Repair of major damage Functions listed in the maintenance Level I can be carried out by the user and are included in the User Guide. Work listed in Level III is carried out at the factory. For this, it is necessary to return the cryostat microtome in its original packing. Note

Maintenance Levels II and III may only be performed by trained service technicians. Unauthorized modification or repair by third party persons will void the warranty.

Field maintenance techniques Service and maintenance work listed in Level II is carried out by a trained service technician. A routine Preventive Maintenance should be performed once a year: DEFECTIVE ASSEMBLIES AND PARTS SHOULD BE RETURNED TO THE FACTORY FOR A DESCRIPTION OF THE MALFUNCTION AS WELL AS THE SERIAL NUMBER OF THE INSTRUMENT IS REQUIRED.

REPAIR OR REPLACEMENT .

 

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Appendix B – PM Procedure Tools required:  Syringe  Allen Keys (different sizes) Parts required:  520110 Link Block Kit  350040 Cryostat Oil  350140 Iso Flex Grease Procedure and Checklist: Step Check the movable parts for good surface quality, dimensional accuracy and wear and tear.

Done □

Check the Cross Roller Bearings for noise as well as the various torques.

□

Check the feed and retraction behavior of the Stepper Motor via a Dial Gauge.

□

Check the Drive Belt Tension and adjust if necessary. Check the smoothness as well as the impact of the Handwheel and adjust if necessary.

□

Check/Set the Real/Defrosting Clock

□

Check Cooling Power (and Temperature Control) according to the Specifications.

□

Clean Condenser

□

Lubricate all movable parts, Cross Roller Bearings with Cryostat Oil the Link Block and the Microtome Columns with ISO FLEX Grease.

□

Empty the Waste Container Bottle.

□

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  Appendix C – Instrument and Service Modules Schematics Appendix C‐1 Instrument Overview

No. 1 2 not shown

Description C-ECU Module Condensing Unit Module Microtome

See Appendix C-2 Appendix C-3 Appendix C-4

 

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  Appendix C‐2 C‐ECU

1

1

C-ECU

435590

Fuse kit

626310

 

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  Appendix C‐3 Microtome and Motor

1 2 3

Microtome Specimen Head cpl. Guidance with socket

556070 550240

4 5 6

Stepper Motor Shaft with bearing Light barrier board

320910 403250 550430

 

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  Appendix C‐4 Condensing Unit

5

3

4

7

2

6

1

1

2 3 4

Condensing Unit assembly(115V) Condensing Unit assembly(230) Fan assembly(115V) Fan assembly(230) Evacuation outlets Condenser

 

Service Manual Cryostat HM525 NX   Cat.No. 391471

435380 5

Quick couplings

435370 6

Staring Relay(115V)

362810

525080 525090 7

Staring Relay(230V) Compressor

362820

 

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Appendix D – Wiring Diagram

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Appendix E – Schematic of the Refrigerating System

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